primescripttm rt reagent kit (TaKaRa)

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TaKaRa primescripttm rt reagent kit
Primescripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89544 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pm42883447-83-7-14
Average 99 stars, based on 89544 article reviews

primescripttm rt reagent kit - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Associations of SLC45A2 variation and MC1R/cAMP-related molecular characteristics with the "cloudy feather" phenotype in Qiangshan Cloud chickens
Article Snippet: cDNA was synthesised following the removal of genomic DNA using the PrimeScriptTM RT reagent Kit with gDNA Eraser (Takara, Japan).

Reverse Transcription:

Article Title: Dioscin Ameliorates Periodontal Bone Destruction by Stimulating Osteogenesis Via the STAT3/ERK Pathway
Article Snippet: Real-time quantitative polymerase chain reaction (qPCR) Total RNA was isolated from cells in each experimental group using TRIzol reagent (Glpbio, Montclair, CA, USA). .. According to the manufacturer’s instructions, RNA was reverse-transcribed into cDNA using the PrimeScriptTM RT Reagent kit (Takara Bio, Kusatsu, Japan). .. qPCR was subsequently performed to detect the expression levels of target genes with SYBR Green Pro Taq HS Premix (Accurate Biotechnology, Changsha, China).

Article Title: Development of green nematicides targeting a repressor of gonad mitosis in Bursaphelenchus xylophilus.
Article Snippet: Background: Bursaphelenchus xylophilus is a worldwide quarantine pest that causes serious damage to the ecological security of pine trees worldwide.. However, the mechanisms involved in mitosis and gamete formation of B. xylophilus have not been reported.. Methods: The expression of Bxy-fbf was determined by qRT-PCR and in situ hybridization.

Article Title: Independent Validation and Refinement of the LAX1 -Regulated Transcriptional Network in Rice Panicle Development.
Article Snippet: .. For reverse transcription, 1 μg of total RNA was utilized with the PrimeScriptTM RT Reagent Kit containing gDNA Eraser (Takara Bio Inc., Kusatsu, Shiga, Japan). .. qRTPCR was conducted with TB Green Premix Ex Taq II (Takara Bio Inc., Kusatsu, Shiga, Japan) on a LightCycler 480II (Roche Diagnostics, Basel, Switzerland).

Article Title: USP25 augments XIAP-mediated signaling to safeguard intestinal homeostasis.
Article Snippet: Nucleotide-binding oligomerization domain containing 2 (NOD2) recognizes conserved bacterial components in the cytoplasm to initiate innate immune responses, and its mutations are closely associated with Crohn’s disease (CD).. Here, we discover that the NOD2 signaling is regulated by the deubiquitinase USP25.. Macrophages lacking USP25 exhibit impaired NOD2 signaling and are defective in cytokine production upon stimulation with muramyl dipeptide (MDP).

Synthesized:

Article Title: ZmDLR9 is required for lateral root development via pre-rRNA processing in maize.
Article Snippet: .. First-strand cDNA was synthesized from the circularized RNA using the PrimeScriptTM RT Reagent Kit (Takara Biomedicals, Otsu, Japan) with gene-specific primers. ..

Article Title: Multi-omics of the nasturtium (Tropaeolum majus) and the parallel evolution of the erucic acid biosynthesis in angiosperms.
Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM RT Reagent Kit with Oligo dT primers according to the manufacturer's protocol (TaKaRa, Dalian, China). ..

cDNA Synthesis:

Article Title: Molecular Characterization and Functional Analysis of Pomacea canaliculata Mos Gene ( PcMos ): A Molecular Switch of Female Fecundity.
Article Snippet: .. First-strand cDNA synthesis was performed using the PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara, Tokyo, Japan). ..

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