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primescripttm rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt reagent kit
    Primescripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+reagent+kit/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pmc13091180-119-13-18
    Average 99 stars, based on 14654 article reviews
    primescripttm rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: A nature-derived, strong adhesive hydrogel microsphere for wet tissue repair: Multifunctional and clinical potential
    Article Snippet: .. When cartilage stem cells were co-cultured with microspheres for 7 days and 14 days, RNAeasyTM kit (Beyotime, China) was used to extract total RNA according to the manufacturer's recommended procedure. cDNA was reverse-transcribed from RNA using PrimeScriptTM RT reagent Kit (TakaRa, Japan). .. TBGreen® pre-mixed ExTaq TM (TakaRa, Japan) was used for quantitative real-time PCR (qPCR) experiments on a Bio-Rad fluorescence quantitative PCR instrument.

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Article Title: In vitro and in vivo anti-piroplasm activity of PBT2 against Theileria annulata and Babesia microti
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. .. PrimeScriptTM RT Reagent Kit (TaKaRa, Japan) was used for reverse transcribed of 1 μg RNA. ..

    Article Title: Metformin alleviates aging-associated periodontitis via NRF2-mediated restoration of the IRE1α dependent unfolded protein response
    Article Snippet: Total RNA was extracted from cells or gingival tissues using RNAiso Plus (9108/9109, Takara). .. Two micrograms of RNA per sample were reverse transcribed into cDNA using the PrimeScriptTM RT Reagent Kit (RR047A, Takara). .. Quantitative real-time PCR was performed using SYBR Green Master Mix (RK21203, Abclonal) on a CFX96 Real-Time PCR Detection System (BIO-RAD) following the manufacturer's protocol.

    Article Title: Microbial-enzymatic co-fermentation of feather meal for bioactive peptide production and its multi-pathway synergistic regulation of intestinal health in broilers
    Article Snippet: .. Total RNA was purified from tissues by means of TRIzol reagent, followed by reverse transcription was conducted to prepare cDNA with the PrimeScriptTM RT Reagent Kit (TaKaRa, Dalian, China); finally, the qPCRs were executed with TB Green® Premix Ex TaqTM II (TaKaRa, Dalian, China). ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..

    Article Title: HIF-2α could be a key regulator of Fe homeostasis in the gut of yellow-feathered broilers
    Article Snippet: Then, Nanodrop ND-1000 (Thermo Fisher, Waltham) was used to analyze the purity of RNA (OD 260/280 ≥ 1.8), and the integrity of RNA was determined by gel electrophoresis. .. Reverse transcription was performed using the PrimeScriptTM RT Reagent Kit (TaKaRa, Ltd., Dalian, China). .. The resulting cDNA was diluted 8-fold and used as a PCR template for real-time qPCR analysis in the CFX ConnectTM RealTime PCR Detection System (Bio‐Rad Laboratories, Inc., California).

    Isolation:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Article Title: MaMYB12 and MaMYB308 antagonistically regulate flavonoid biosynthesis in mulberry ( Morus alba ): Implications for functional food ingredient development
    Article Snippet: .. Total RNA was isolated using a Plant Total RNA Extraction Kit, followed by DNase I treatment. cDNA synthesis was performed with the PrimeScriptTM RT reagent Kit (TaKaRa Bio, Dalian, China). qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR system using SYBR® Premix Ex TaqTM II (TaKaRa Bio, Dalian, China). ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..

    Expressing:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Article Title: MaMYB12 and MaMYB308 antagonistically regulate flavonoid biosynthesis in mulberry ( Morus alba ): Implications for functional food ingredient development
    Article Snippet: .. Total RNA was isolated using a Plant Total RNA Extraction Kit, followed by DNase I treatment. cDNA synthesis was performed with the PrimeScriptTM RT reagent Kit (TaKaRa Bio, Dalian, China). qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR system using SYBR® Premix Ex TaqTM II (TaKaRa Bio, Dalian, China). ..

    Article Title: Human 3D liver spheroids support productive infection of a novel tick-borne phenuivirus
    Article Snippet: Albumin (ALB) and urea were quantified with the Human Serum Albumin ELISA Kit (4 A BIOTECH, China) and Urea Assay Kit E2020 (Applygen Technologies, China), respectively [ , ]. .. Total RNA was extracted from spheroids (72 hpi) using the RNA-easy Isolation Reagent (Vazyme, China) and reverse transcribed (PrimeScriptTM RT reagent Kit with gDNA Eraser,TaKaRa, Japan). qPCR was conducted with TB Green® Premix Ex TaqTM II (TaKaRa, Japan) under the following conditions: 95 °C for 30 s; 40 cycles of 95 °C for 5 s and 60 °C for 30 s. Primers are listed in Table S1. ..

    RNA Extraction:

    Article Title: MaMYB12 and MaMYB308 antagonistically regulate flavonoid biosynthesis in mulberry ( Morus alba ): Implications for functional food ingredient development
    Article Snippet: .. Total RNA was isolated using a Plant Total RNA Extraction Kit, followed by DNase I treatment. cDNA synthesis was performed with the PrimeScriptTM RT reagent Kit (TaKaRa Bio, Dalian, China). qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR system using SYBR® Premix Ex TaqTM II (TaKaRa Bio, Dalian, China). ..

    cDNA Synthesis:

    Article Title: MaMYB12 and MaMYB308 antagonistically regulate flavonoid biosynthesis in mulberry ( Morus alba ): Implications for functional food ingredient development
    Article Snippet: .. Total RNA was isolated using a Plant Total RNA Extraction Kit, followed by DNase I treatment. cDNA synthesis was performed with the PrimeScriptTM RT reagent Kit (TaKaRa Bio, Dalian, China). qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR system using SYBR® Premix Ex TaqTM II (TaKaRa Bio, Dalian, China). ..

    Quantitative RT-PCR:

    Article Title: MaMYB12 and MaMYB308 antagonistically regulate flavonoid biosynthesis in mulberry ( Morus alba ): Implications for functional food ingredient development
    Article Snippet: .. Total RNA was isolated using a Plant Total RNA Extraction Kit, followed by DNase I treatment. cDNA synthesis was performed with the PrimeScriptTM RT reagent Kit (TaKaRa Bio, Dalian, China). qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR system using SYBR® Premix Ex TaqTM II (TaKaRa Bio, Dalian, China). ..

    Purification:

    Article Title: Microbial-enzymatic co-fermentation of feather meal for bioactive peptide production and its multi-pathway synergistic regulation of intestinal health in broilers
    Article Snippet: .. Total RNA was purified from tissues by means of TRIzol reagent, followed by reverse transcription was conducted to prepare cDNA with the PrimeScriptTM RT Reagent Kit (TaKaRa, Dalian, China); finally, the qPCRs were executed with TB Green® Premix Ex TaqTM II (TaKaRa, Dalian, China). ..



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